![]() Samples should be transferred directly to the laboratory without delay and should not be exposed to extreme heat or cold.Cultures of specimens grown on selective media should, therefore, be compared with specimens cultured on nonselective media to obtain additional information and help ensure recovery of potential pathogens. A single medium is rarely adequate for detecting all organisms of potential significance in a specimen.Consult appropriate texts for detailed information and recommended procedures. Morphological, biochemical, and/or serological tests should be performed for final identification. For identification, organisms must be in pure culture.Some Proteus strains will give black-centered colonies on XLD Agar.gallinarum may form red colonies without black centers, thus resembling Shigella species. Incubation in excess of 48 hours may lead to false-positive results.Red, false-positive colonies may occur with some Proteus and Pseudomonas species.Note: It is advisable not to prepare large volumes, which will require prolonged heating and may produce precipitate. ![]()
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